Fig. 3

Graphs showing a decrease in X. fastidiosa acquisition by paratransgenic GWSSs. P. agglomerans was painted on grape stems after mixing with guar gum. PA(WT) - wild type P. agglomerans; PA(HlyA) - P. agglomerans expressing HlyA secretion signal only; PA(Melittin) - P. agglomerans expressing melittin conjugated to HlyA; PA(SLM) - P. agglomerans expressing SLM conjugated to HlyA. The GWSSs were allowed to feed on Pantoea-painted plants for 48 h before putting them in a cage containing X. fastidiosa-infected plants for 48 h. Subsequently the GWSSs were collected and two GWSSs were caged per single naive grape plant for 24 h. These GWSSs were surface sterilized and X. fastidiosa presence was assayed using rt-PCR. a X. fastidiosa CFUs per insect head; b Percent of GWSSs carrying X. fastidiosa. These are pooled results from two independent experiments