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Fig. 2 | BMC Biotechnology

Fig. 2

From: A paratransgenic strategy to block transmission of Xylella fastidiosa from the glassy-winged sharpshooter Homalodisca vitripennis

Fig. 2

a Western blot showing secretion and accumulation of melittin and SLM conjugated to HlyA secretion signal by transformed P. agglomerans lines in spent media. Spent media from transformed P. agglomerans lines were concentrated using Micron 10 kDa filters. Concentrated spent medium was tested using an anti-E-tag antibody. Lane 1: ladder; lane 2: Wild type P. agglomerans; lane 3: HlyA secretion signal only; lane 4: melittin conjugated to HlyA secretion signal; lane 5: SLM conjugated to HlyA secretion signal. b, c Western blots showing secretion and accumulation of melittin and SLM conjugated to HlyA secretion signal by transformed P. agglomerans lines in the GWSS gut. Extracts from homogenized GWSSs were tested for presence of AMPs using an anti-E-tag antibody. b Lane 1: ladder; lane 2: GWSS fed on P. agglomerans expressing melittin conjugated to HlyA secretion signal; lane 3: GWSS fed on wild type P. agglomerans (c) Lane 1: ladder; lane 2: GWSS fed on P. agglomerans expressing SLM; lane 3: GWSS fed on wild type P. agglomerans. Five insects were tested individually for accumulation of SLM and melittin, and two insects were found positive for presence of both AMPs

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