Fig. 2
From: Acetosyringone treatment duration affects large T-DNA molecule transfer to rice callus
T-strand-immunoprecipitation and qPCR assay in Agrobacterium cells after AS induction. a Immunoblot analysis to examine the antibody against VirD2. The GST-VirD2, but not GST, can be detected by the antibody against VirD2. GST-VirD2 bands are indicated by the red arrow. Increased amount of GST-VirD2 (2.5 ng and 10 ng) is indicated by the black triangle. b Immunoblot analysis of VirD2 expression level (upper panel) in Agrobacterium cells after various hours of AS induction. Total protein is detected with Coomassie brilliant blue (CBB) staining (lower panel). c Comparison of the fold change of immunoprecipitated (IPed) T-strands between B5 and B50 tested with the left border marker. d Comparison of the fold change of IPed T-strands between B5 and B50 tested with the right border marker. e Comparison of the fold change of IPed T-strands between C5 and B5 tested with the left border marker. f Comparison of the fold change of IPed T-strands between pC5 and pB5 tested with the right border marker. Data represent means and standard errors (n = 3). ***p < 0.001, **p < 0.01, *p < 0.05 show significance level in Student’s t test