Skip to main content
Fig. 1 | BMC Biotechnology

Fig. 1

From: Expression of novel fusion antiviral proteins ricin a chain-pokeweed antiviral proteins (RTA-PAPs) in Escherichia coli and their inhibition of protein synthesis and of hepatitis B virus in vitro

Fig. 1

Medium Optimization and Protein Purification. a Medium optimization for Ricin-PAPS1 (RP1) expression. Three different growth media including M9 (M9), Luria Bertani (LB) and terrific broth (TB) were tested for Ricin-PAPS1 expression at 30 °C. Soluble lysate (Sol) and inclusion body (IB) from each sample were analyzed by SDS PAGE and visualized by Coomassie blue staining. b Validation of purified Ricin-PAPS1 protein. Recombinant Ricin-PAPS1 was produced in 1 L of culture that was induced with the optimized condition (LB medium with 1 mM IPTG at 30 °C for 4 h) and purified from inclusion bodies through gel filtration before refolding, concentration and dialysis. The resulting protein of approx. 60.5 kDa was > 90% purity determined by SDS-PAGE

Back to article page

BMC Biotechnology

ISSN: 1472-6750

Contact us