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Table 1 NicA2 substrate specificity. Activity using the various compounds as substrates measured in an Amplex Red assay. Activities listed in percentages relative to that of nicotine. GABA; γ-amino-N-butyric acid, NAD; β-nicotinamide adenine dinucleotide

From: The nicotine-degrading enzyme NicA2 reduces nicotine levels in blood, nicotine distribution to brain, and nicotine discrimination and reinforcement in rats

Compound % Activity
Nicotine 100
Anatabine 49
Nicotine-1’-N-oxide 44
Nornicotine 40
Anabasine < 5
Myosmine < 5
Norepinephrine < 5
Dopamine < 5
Serotonin < 5
GABA < 5
Choline < 5
Acetylcholine < 5
Nicotinamide < 5
NAD < 5
L-Glutamate < 5
Cytisine < 5
Mecamylamine < 5
Varenicline < 5
Bupropion < 5