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Fig. 2 | BMC Biotechnology

Fig. 2

From: The nicotine-degrading enzyme NicA2 reduces nicotine levels in blood, nicotine distribution to brain, and nicotine discrimination and reinforcement in rats

Fig. 2

Quenching of NicA2 activity in blood in vitro by MeOH. MeOH was added in vitro to blood containing 40 ng/ml nicotine before or after adding NicA2. Prior addition of MeOH completely quenched NicA2 activity (no difference from BSA control). In contrast, adding MeOH after an approximately 10 s exposure of nicotine to NicA2 in vitro allowed degradation of nicotine to below the limit of assay detection. *** p < 0.001 using two-tailed unpaired t tests with Welch’s correction. Mean ± SD, n = 8/group

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