Fig. 2
From: pXST, a novel vector for TA cloning and blunt-end cloning
Gel electrophoretogram of colony PCR and restriction. a Lane 1–10 shows the results of colony PCR from ten random clones, which were selected for the detecting the insert orientation of XcmI-ccdB-XcmI cassette. The light bands indicate positive clones, in which the ccdB coding sequence is in the downstream of LAC promoter. b Plasmid vector pXST was digested with SmaI (lane 1) and XcmI (lane 2–3)