Fig. 7

Enzyme activity of CFE from GE39 and GE40 expressing E. coli BL21(DE3) cultures. CFE was incubated at a pH of 6.0 on a series of chromogenic substrates and incubated for 24 h at 60 °C in a rotary shaker (180 RPM). Degradation of the substrate by cellulolytic activity was measured by colorimetric measurements of the solubilized chromogenic oligosaccharides at 595 nm