Fig. 3From: Engineering Geobacillus thermodenitrificans to introduce cellulolytic activity; expression of native and heterologous cellulase genesOverview C. thermocellum constructs for expression in G. thermodenitrificans T12. Numbers indicate start and end amino acid residues of the original cellulases as deposited in the UniprotKB database. Green: promoter sequence derived from the uracil phosphoribosyltransferase gene native to G. thermodenitrificans T12, yellow: signal peptide sequence derived from the endoxylanse encoding gene (GtxynA1) native to G. thermodenitrificans T12, purple: carbohydrate binding domain encoded by celK from C. thermocellum, blue: linker domain of the celK gene from C. thermocellum., red: catalytic domains of the indicated genes, orange: G. thermodenitrificans codon harmonized sequence encoding the catalytic domain of celSBack to article page