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Fig. 3 | BMC Biotechnology

Fig. 3

From: Engineering Geobacillus thermodenitrificans to introduce cellulolytic activity; expression of native and heterologous cellulase genes

Fig. 3

Overview C. thermocellum constructs for expression in G. thermodenitrificans T12. Numbers indicate start and end amino acid residues of the original cellulases as deposited in the UniprotKB database. Green: promoter sequence derived from the uracil phosphoribosyltransferase gene native to G. thermodenitrificans T12, yellow: signal peptide sequence derived from the endoxylanse encoding gene (GtxynA1) native to G. thermodenitrificans T12, purple: carbohydrate binding domain encoded by celK from C. thermocellum, blue: linker domain of the celK gene from C. thermocellum., red: catalytic domains of the indicated genes, orange: G. thermodenitrificans codon harmonized sequence encoding the catalytic domain of celS

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