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Fig. 1 | BMC Biotechnology

Fig. 1

From: Engineering Geobacillus thermodenitrificans to introduce cellulolytic activity; expression of native and heterologous cellulase genes

Fig. 1

Overview of the workflow for detecting novel cellulases from a G. thermodenitrificans metagenome. From compost a total of 73 G. thermodenitrificans strains were isolated of which the genomic DNA was pooled and subjected to shotgun sequencing. By screening the assembled metagnome data on proteins matching against HMM profiles of all known glycoside hydrolase families that contain endoglucanase and/or exoglucanases we identified several novel putative cellulases. The genes encoding these putative cellulases were used to create expression constructs in both E. coli and G. thermodenitrificans T12. The supernatant and CFE of these cultures were then subject to activity assays against a variety of substrates

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