Table 1 Primer sequences used to produce fusion proteins
From: Tetanus toxin fragments and Bcl-2 fusion proteins: cytoprotection and retrograde axonal migration
Primer name | Sequence | Modified sequence(s) |
|---|---|---|
Bcl2 sens | 5’-GATCTAGAATGGACTACAAAGACGACGACGACAAGGCTAGCATGGCGCACGCTGGGAGA-3′ | XbaI, start codon, FLAG, NheI |
Bcl-2 rev | 5’-GGGTCGACTTACTCGAGAATCTTGTGGCCCAGATAGGC-3’ | XhoI, stop codon, SalI |
TTC sens | 5’-AAACGCGTATGGACTACAAAGACGACGACGACAAGCTCGAGATGAAAAATCTGGATTGTT-3’ | MluI, start codon, FLAG, XhoI |
TTC rev | 5’-CAGCGGCCGCTCATCTAGAAATATCATTTGTCCATCCTTC-3’ | XbaI, stop codon, NotI |
TTH sens | 5’-AGACTCGAGTCATTAACAGATTTAGGAGGA-3’ | XhoI |
TTH rev | similar to TTC rev | Â |