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Table 1 Primer sequences used to produce fusion proteins

From: Tetanus toxin fragments and Bcl-2 fusion proteins: cytoprotection and retrograde axonal migration

Primer name

Sequence

Modified sequence(s)

Bcl2 sens

5’-GATCTAGAATGGACTACAAAGACGACGACGACAAGGCTAGCATGGCGCACGCTGGGAGA-3′

XbaI, start codon, FLAG, NheI

Bcl-2 rev

5’-GGGTCGACTTACTCGAGAATCTTGTGGCCCAGATAGGC-3’

XhoI, stop codon, SalI

TTC sens

5’-AAACGCGTATGGACTACAAAGACGACGACGACAAGCTCGAGATGAAAAATCTGGATTGTT-3’

MluI, start codon, FLAG, XhoI

TTC rev

5’-CAGCGGCCGCTCATCTAGAAATATCATTTGTCCATCCTTC-3’

XbaI, stop codon, NotI

TTH sens

5’-AGACTCGAGTCATTAACAGATTTAGGAGGA-3’

XhoI

TTH rev

similar to TTC rev