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Table 1 Primer sequences used to produce fusion proteins

From: Tetanus toxin fragments and Bcl-2 fusion proteins: cytoprotection and retrograde axonal migration

Primer name Sequence Modified sequence(s)
Bcl2 sens 5’-GATCTAGAATGGACTACAAAGACGACGACGACAAGGCTAGCATGGCGCACGCTGGGAGA-3′ XbaI, start codon, FLAG, NheI
Bcl-2 rev 5’-GGGTCGACTTACTCGAGAATCTTGTGGCCCAGATAGGC-3’ XhoI, stop codon, SalI
TTC sens 5’-AAACGCGTATGGACTACAAAGACGACGACGACAAGCTCGAGATGAAAAATCTGGATTGTT-3’ MluI, start codon, FLAG, XhoI
TTC rev 5’-CAGCGGCCGCTCATCTAGAAATATCATTTGTCCATCCTTC-3’ XbaI, stop codon, NotI
TTH sens 5’-AGACTCGAGTCATTAACAGATTTAGGAGGA-3’ XhoI
TTH rev similar to TTC rev