Variants | 15 °C | 50 °C | ||||||
---|---|---|---|---|---|---|---|---|
Km (mM) | k cat | kcat/Km (s− 1·M− 1) | Fold (◊) | Km (mM) | k cat | kcat/Km (s− 1·M− 1) | Fold (◊) | |
(s−1) | (s−1) | |||||||
wt | 0.16 ± 0.02 | 102 ± 03 | 0.64E × 106 | 1.00 | 0.23 ± 0.018 | 609 ± 14 | 2.65E × 106 | 1.00 |
P9S | 0.41 ± 0.09 | 362 ± 43 | 0.88E × 106 | 1.37 | 0.43 ± 0.045 | 829 ± 81 | 1.97E × 106 | 0.74 |
A1G/K27Q | 0.20 ± 0.04 | 373 ± 27 | 1.87E × 106 | 2.92 | 0.36 ± 0.081 | 1719 ± 161 | 4.78E × 106 | 1.83 |
A38V | 0.13 ± 0.02 | 176 ± 11 | 1.35E × 106 | 2.11 | 0.20 ± 0.096 | 952 ± 123 | 4.76E × 106 | 1.79 |
A116T | 0.22 ± 0.05 | 262 ± 22 | 1.19E × 106 | 1.86 | 0.25 ± 0.040 | 778 ± 71 | 3.11E × 106 | 1.17 |
T162I | 0.17 ± 0.03 | 300 ± 20 | 1.76E × 106 | 2.75 | 0.26 ± 0.026 | 1149 ± 53 | 4.41E × 106 | 1.66 |
S182R | 0.19 ± 0.03 | 259 ± 18 | 1.36E × 106 | 2.13 | 0.23 ± 0.50 | 897 ± 102 | 3.90E × 106 | 1.47 |
T351S | 0.31 ± 0.05 | 283 ± 24 | 0.91E × 106 | 1.42 | 0.25 ± 0.053 | 989 ± 97 | 3.96E × 106 | 1.49 |
P9S/T162I | 0.30 ± 0.013 | 450 ± 10.2 | 1.50E × 106 | 2.34 | 0.26 ± 0.009 | 1169 ± 22 | 4.50E × 106 | 1.70 |
P9S/K27Q | 0.35 ± 0.035 | 645 ± 28.5 | 1.84E × 106 | 2.88 | 0.32 ± 0.015 | 1555 ± 40 | 4.86E × 106 | 1.83 |
K27Q/T162I | 0.21 ± 0.013 | 288 ± 5.9 | 1.37E × 106 | 2.14 | 0.25 ± 0.018 | 1092 ± 22 | 4.37E × 106 | 1.65 |
P9S/K27Q/T162I | 0.34 ± 0.016 | 579 ± 19.9 | 1.70E × 106 | 2.65 | 0.34 ± 0.016 | 1596 ± 47 | 4.69E × 106 | 1.77 |