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Table 1 Nucleotide substitutions in the DHAP gene in the mutation library

From: Engineering Bacillus pumilus alkaline serine protease to increase its low-temperature proteolytic activity by directed evolution

Mutation types

Nucleotide change

Number

Percentage (%)

Transitions

A → G

11

20.37

G → A

9

16.67

C → T

16

29.63

T → C

11

20.37

Total

47

87.04

Transversions

G → C

1

1.85

C → G

2

3.70

C → A

3

5.56

A → T

1

1.85

Total

7

12.96