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Table 1 Nucleotide substitutions in the DHAP gene in the mutation library

From: Engineering Bacillus pumilus alkaline serine protease to increase its low-temperature proteolytic activity by directed evolution

Mutation types Nucleotide change Number Percentage (%)
Transitions A → G 11 20.37
G → A 9 16.67
C → T 16 29.63
T → C 11 20.37
Total 47 87.04
Transversions G → C 1 1.85
C → G 2 3.70
C → A 3 5.56
A → T 1 1.85
Total 7 12.96