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Fig. 3 | BMC Biotechnology

Fig. 3

From: Engineering Bacillus pumilus alkaline serine protease to increase its low-temperature proteolytic activity by directed evolution

Fig. 3

Temperature-dependent caseinolytic activity of the wild-type and combined variants of the alkaline serine protease DHAP. The activity assay was performed in a 400 μL reaction mixture (borate/NaOH buffer, pH 9.6, 2 mM CaCl2) containing 1% casein as substrate and 0.2 μg of enzyme for 10 min. The data are expressed as the average value from three independent experiments with the error bars shown

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