Fig. 1

Temperature-dependent caseinolytic activity of the wild-type and variants of the alkaline serine protease DHAP. An activity assay was performed in a 400 μL reaction mixture (borate/NaOH buffer, pH 9.6, 2 mM CaCl₂) containing 1% casein as substrate and 0.2 μg enzyme for 10 min. The data are expressed as the average value from three independent experiments with the experimental deviation shown