Fig. 8

Binding of ANRE and normal human CSF and sera to 293T-ATD cells by immunofluorescence. 293T-ATD cells were stained with (a) ANRE patient CSF, (b) normal human CSF, (c) ANRE patient sera, and (d) normal human sera. Matched pairs include ANRE patient 10–071 (a, c) and normal subjects 10–123 and 10–551 (d, d). CSF were tested at 1:20 dilution; sera at 1:100. Human IgG binding is shown in red. Nuclei were stained with DAPI (blue) and the cells were visualized by confocal microscopy. Scale bars = 10 μm