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Fig. 1 | BMC Biotechnology

Fig. 1

From: Membrane-bound and soluble forms of an NMDA receptor extracellular domain retain epitopes targeted in auto-immune encephalitis

Fig. 1

Structure and expression of the NR1 Amino Terminal Domain (ATD) fusion protein on 293 T cells. (a, b) The ATD fusion protein consists of the entire 561 N-terminal amino acid extracellular domain, which includes the bi-lobed NR1 ATD, fused in sequence to the Myc tag, the 6XHIS tag, the Tobacco Etch Virus (TEV) protease site, and the platelet derived growth factor receptor (PDGFR) transmembrane domain. The cartoon is not strictly drawn to scale. Color code: blue and green; extracellular GluN1 bi-lobed domain; purple, Myc tag; light green, 6XHIS tag; orange, TeV protease site; brown, PDGFR transmembrane domain. (c, d) Expression of the ATD fusion protein on the surface of 293T cells was analyzed by flow cytometry with a commercial anti-GluN1 mAb either alone (c) or with an anti-Myc tag mAb (d)

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