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Table 2 Analysis of the bidirectional DNA walking method, including the Cry-F and Cry-R walking direction, applied on GM events (2000 HGEs) containing the CryAb/c genes, using the four different mixes of DRT primers (A-D)

From: Development and validation of an integrated DNA walking strategy to detect GMO expressing cry genes

  Cry-F Cry-R
Bt11 maize + +
  A B C D A B C D
  550 500 650
950
1250
450
1000
1400*
1800
400*
800
> 2000 500
1000
1400
550
1300
1600
MON810 maize + +
A B C D A B C D
550*
850
1300
650   400 250* 770 350
500
260
T304–40 cotton + +
A B C D A B C D
550
800
350 450
500
600
800*
1200
400
600
550 200 200
450
550
200
450
700*
MON531 cotton + +
A B C D A B C D
550 500* 950 450
1000
1700
1900
> 2000
350
600
1000
1400
  750*
1400
 
MON87701 soybean + +
A B C D A B C D
550 450
530
500
1000
> 2000
430
1000*
1700
> 2000
320
450
550
860
550
700
1000
1800*
550
660
700
1000
300
470
850
KeFeng-6 rice + +
A B C D A B C D
550 440
520
720
970
250
470
950
790
430
980*
1650
700
1000
500
750
1100
370
500
800
1200
950*
1500
1800
MON15985 cotton + +
A B C D A B C D
550 500 900* 430
800
1000
> 1500
350
600
1000
1500
> 2000 500
800*
1000
1200
220
281–24-236 × 3006–210-23 cotton + +
A B C D A B C D
200
450
900
1000
  550 420* 300
550
520 550
750*
 
MON89034 maize + +
A B C D A B C D
600
850
1000*
660   420
500
650
900 300*
600
550 750
Bt176 maize + +
A B C D A B C D
550 250 600 400*
550
670
1000
350
500
300
600
350
450*
500
850
1000
1700
  1. For each result, the experiment was carried out in triplicate. The detection of the targets is symbolized by + (amplicons observed in each repetition) or (+) (amplicons not observed in each repetition). The approximate size of amplicons is indicated in base-pair for each DRT primers. The amplicons observed in each repetition (3/3) are indicated in bold while the amplicons obtained not in each repetition (1–2/3) are indicated in italic. The sequenced amplicons are indicated by an asterisk