Lignocellulose integration to 1G-ethanol process using filamentous fungi: fermentation prospects of edible strain of Neurospora intermedia

Table 4 Fermentation profile by N. intermedia on integrated fermentation substrate using wheat straw pretreated with dilute phosphoric acid (H₁₉₀- acid concentration of 1.75% (w/v), duration of 10 min, and temperature of 190 ± 2 °C) and thin stillage (T_HS) mixture

Substrate combination (%w/v pretreated wheat straw + thin stillage)	Inoculation mode	Mycelial growth form	Dry Biomass (g/L)	Ethanol max (g/L)	Ethanol max- Fermentation time (h)
7% solid loading
WS_S + T_HS	Pellets	–	–	–	–
WS_S + T_HS	Filamentous	Filamentous	7.88 ± 1.28	6.25 ± 0.77	72
WS_S	Pellets	–	–	–	–
WS_S	Filamentous	–	–	–	–
WS_L+ T_HS	Pellets	–	–	–	–
WS_L+ T_HS	Filamentous	Filamentous	6.25 ± 0.18	9.22 ± 1.02	72
WS_L	Pellets	–	–	–	–
WS_L	Filamentous	–	–	–	–
3.5% solid loading
WS_s + T_HS	Pellets	Filamentous	9.22 ± 0.57	6.18 ± 0.85	72
WS_s + T_HS	Filamentous	Filamentous	6.87 ± 0.19	5.54 ± 0.15	72
WS_s	Pellets	–	–	–	–
WS_s	Filamentous	–	–	–	–
WS_L+ T_HS	Pellets	Filamentous	7.37 ± 0.88	5.96 ± 0.51	72
WS_L+ T_HS	Filamentous	Filamentous	10.95 ± 1.10	6.47 ± 0.79	72
WS_L	Pellets	Pellets	3.91 ± 0.11	0.11 ± 0.00	48
WS_L	Filamentous	Filamentous	3.01 ± 0.07	0.15 ± 0.04	72

WS_S, wheat straw slurry; WS_L, wheat straw liquid hydrolysate after enzymatic hydrolysis