Lignocellulose integration to 1G-ethanol process using filamentous fungi: fermentation prospects of edible strain of Neurospora intermedia

Table 3 Fermentation profile by N. intermedia on integrated fermentation substrate using wheat straw pretreated with dilute phosphoric acid (H₁₉₅- acid concentration of 1.2% (w/v), duration of 7 min, and temperature of 195 ± 2 °C) and thin stillage (T_HS) mixture

Substrate combination (%w/v pretreated wheat straw + thin stillage)	Inoculation mode	Mycelial growth form	Dry Biomass (g/L)	Ethanol max (g/L)	Ethanol max- Fermentation time (h)
7% solid loading
WS_S + T_HS	Pellets	Filamentous	21.49 ± 1.18	7.18 ± 0.87	72
WS_S + T_HS	Filamentous	Filamentous	17.78 ± 0.47	8.04 ± 0.07	48
WS_S	Pellets	–	–	–	–
WS_S	Filamentous	–	–	–	–
WS_L+ T_HS	Pellets	Filamentous	13.61 ± 1.08	8.45 ± 0.77	48
WS_L+ T_HS	Filamentous	Filamentous	6.62 ± 0.24	4.52 ± 0.78	48
7% WS_L	Pellets	–	–	–	–
7% WS_L	Filamentous	–	–	–	–
3.5% solid loading
WS_s + T_HS	Pellets	Filamentous	13. 88 ± 0.82	7.89 ± 0.71	48
WS_s + T_HS	Filamentous	Filamentous	8.52 ± 0.62	7.42 ± 0.11	48
WS_s	Pellets	Pellets	16.84 ± 1.28	0.13 ± 0.08	24
WS_s	Filamentous	Filamentous	20.48 ± 1.12	0.31 ± 0.04	48
WS_L+ T_HS	Pellets	Filamentous	8.94 ± 0.71	8.35 ± 1.01	48
WS_L+ T_HS	Filamentous	Filamentous	13.61 ± 1.02	7.79 ± 0.91	48
WS_L	Pellets	Pellets	4.73 ± 0.18	1.69 ± 0.87	48
WS_L	Filamentous	Filamentous	11.2 ± 0.45	0.13 ± 0.02	24

WS_S, wheat straw slurry; WS_L, wheat straw liquid hydrolysate after enzymatic hydrolysis

ISSN: 1472-6750