The production of a recombinant tandem single chain fragment variable capable of binding prolamins triggering celiac disease

Table 2 Strain physiological parameters of E. coli BL21(DE3) producing tscFv IBs

	cultivation time	specific glucose uptake rate	growth rate	Biomass concentration	C-balance	specific product titer	volumetric product titer
	[h]	qs Gluc [g/g/h]	μ [h⁻¹]	g DCW/L		[mg/g]	[g/L]
Batch	0–6.7	0.62	0.6	8.13	0.95
Fed-Batch	6.7–22.4	0.29	0.09	47.60	0.89
Induced Fed-Batch	22.4–32.4	0.20	0.05	56.15	1.01	40.90	2.30

ISSN: 1472-6750