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Fig. 7 | BMC Biotechnology

Fig. 7

From: The production of a recombinant tandem single chain fragment variable capable of binding prolamins triggering celiac disease

Fig. 7

Competitive ELISA of refolded tscFv and flours considered as safe (a) as well as flours known to trigger CD (b). The ability of flours from different grains to replace refolded tscFv from immobilized PT-gliadin was tested. The tscFv was applied in a concentration of 8 μg/ml with flours in predefined total protein concentrations (0, 0.0075, 75, 125, 250, 500 and 1000 μg/mL). The relative signal in % is shown. 100% signal corresponds to the signal obtained with tscFv without any flour

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