Table 1 Strains and plasmids used and constructed in this study
Strain or plasmid | Relevant characteristics | Sources of reference |
|---|---|---|
Strains | ||
Sm320 | Sinorhizobium meliloti 320, VB12 producing strain. | [22] |
Sm320-L6 | Sm320 derivative, carrying plasmid pBR-OhemE to reduce production capacity | This study |
Sm320-N8 | Sm320 derivative, knocking out cobI gene | This study |
Sm320-Mybi | Sm320 derivative, carrying plasmid pBR- SYB-lacI and plasmid pMB-gfp was used in the ARTP treat | This study |
MT616 | MT607/pRK600; Escherichia coli helper strain for mobilizing RK2/RP4-derived plasmids | [24] |
Plasmid | ||
pJQ200sk | Vector for construction of deletions, sacB,GmR | [31] |
pMB393 | Broad-host-range vector, PtauA promoter,AmpR | [32] |
pBHR1 | Broad-host-range vector, Derivative of PBBR122,KmR | This lab |
pBR- EC-butB | pBHR1 plasmid carrying EC-butB riboswitch were cloned in front of a gfp reporter gene driven by the constitutive promoter PmelA | This study |
pBR- PF-cbiB | pBHR1 plasmid carrying PF-cbiB riboswitch were cloned in front of a gfp reporter gene driven by the constitutive promoter PmelA | This study |
pBR- SM-bluB | pBHR1 plasmid carrying SM-bluB riboswitch were cloned in front of a gfp reporter gene driven by the constitutive promoter PmelA | This study |
pBR- SM-cobU | pBHR1 plasmid carrying SM-cobU riboswitch were cloned in front of a gfp reporter gene driven by the constitutive promoter PmelA | This study |
pBR- MLO-bluB | pBHR1 plasmid carrying MLO-bluB riboswitch were cloned in front of a gfp reporter gene driven by the constitutive promoter PmelA | This study |
pBR- MLO-metE | pBHR1 plasmid carrying MLO-metE riboswitch were cloned in front of a gfp reporter gene driven by the constitutive promoter PmelA | This study |
pBR- SY-btuB | pBHR1 plasmid carrying SY-btuB riboswitch were cloned in front of a gfp reporter gene driven by the constitutive promoter PmelA | This study |
pBR- BI-cbiW | pBHR1 plasmid carrying BI-cbiW riboswitch were cloned in front of a gfp reporter gene driven by the constitutive promoter PmelA | This study |
pBR- SYB-lacI | pBHR1 plasmid carrying SY-btuB riboswitch were cloned in front of a lacI reporter | This study |
pMB-gfp | pMB393 containing lacO and carrying gfp reporter | This study |
pBR-OhemE | pBHR1 plasmid carrying hemE gene to reduce production capacity | This study |