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Table 1 Strains and plasmids used and constructed in this study

From: Engineering a vitamin B12 high-throughput screening system by riboswitch sensor in Sinorhizobium meliloti

Strain or plasmid Relevant characteristics Sources of reference
Strains   
 Sm320 Sinorhizobium meliloti 320, VB12 producing strain. [22]
 Sm320-L6 Sm320 derivative, carrying plasmid pBR-OhemE to reduce production capacity This study
 Sm320-N8 Sm320 derivative, knocking out cobI gene This study
 Sm320-Mybi Sm320 derivative, carrying plasmid pBR- SYB-lacI and plasmid pMB-gfp was used in the ARTP treat This study
 MT616 MT607/pRK600; Escherichia coli helper strain for mobilizing RK2/RP4-derived plasmids [24]
Plasmid   
 pJQ200sk Vector for construction of deletions, sacB,GmR [31]
 pMB393 Broad-host-range vector, PtauA promoter,AmpR [32]
 pBHR1 Broad-host-range vector, Derivative of PBBR122,KmR This lab
 pBR- EC-butB pBHR1 plasmid carrying EC-butB riboswitch were cloned in front of a gfp reporter gene driven by the constitutive promoter PmelA This study
 pBR- PF-cbiB pBHR1 plasmid carrying PF-cbiB riboswitch were cloned in front of a gfp reporter gene driven by the constitutive promoter PmelA This study
 pBR- SM-bluB pBHR1 plasmid carrying SM-bluB riboswitch were cloned in front of a gfp reporter gene driven by the constitutive promoter PmelA This study
 pBR- SM-cobU pBHR1 plasmid carrying SM-cobU riboswitch were cloned in front of a gfp reporter gene driven by the constitutive promoter PmelA This study
 pBR- MLO-bluB pBHR1 plasmid carrying MLO-bluB riboswitch were cloned in front of a gfp reporter gene driven by the constitutive promoter PmelA This study
 pBR- MLO-metE pBHR1 plasmid carrying MLO-metE riboswitch were cloned in front of a gfp reporter gene driven by the constitutive promoter PmelA This study
 pBR- SY-btuB pBHR1 plasmid carrying SY-btuB riboswitch were cloned in front of a gfp reporter gene driven by the constitutive promoter PmelA This study
 pBR- BI-cbiW pBHR1 plasmid carrying BI-cbiW riboswitch were cloned in front of a gfp reporter gene driven by the constitutive promoter PmelA This study
 pBR- SYB-lacI pBHR1 plasmid carrying SY-btuB riboswitch were cloned in front of a lacI reporter This study
 pMB-gfp pMB393 containing lacO and carrying gfp reporter This study
 pBR-OhemE pBHR1 plasmid carrying hemE gene to reduce production capacity This study