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Table 2 Effects of metal cations and reagents on the recombinant Xln-DT activity

From: Characterization of a novel thermostable and xylose-tolerant GH 39 β-xylosidase from Dictyoglomus thermophilum

Cation of reagent

Relative enzyme activity (%)

1 mM

5 mM

Contrast

100

100

PMSF

112.4

114.5

EDTA

94.3

90.5

Al3+

101.5

98.1

Cu2+

48.1

39.9

Zn2+

84.2

83.4

Ca2+

98.3

87.1

Fe2+

155.8

327.5

Fe3+

99.8

199.3

Na+

95.8

99.5

K+

101.3

101.4

Li+

92.3

91.1

Mg2+

102.8

97.1

NH4+

99.3

96.2

Mn2+

98.3

115.8

Ba2+

92.8

91.9

Hg2+

35.3

35.1

Co2+

95.8

89.6

Ni2+

108.2

89.2

  1. Values shown were the mean of duplicate experiments, and the variation about the mean was below 5%