Characterization of a novel thermostable and xylose-tolerant GH 39 β-xylosidase from Dictyoglomus thermophilum

BMC Biotechnology

Table 1 Purification of recombinant protein Xln-DT

Purification step	Total activity(U)	Total protein(mg)	Specific activity (U/mg)	Yield (%)	Fold purification
Culture extract	539.1	187.2	2.88	100	1
Heat treatment^a	392.3	67.3	5.83	72.8	2.02
Ni affinity chromatography	283.5	33.5	8.46	52.6	2.94

Substrate for Xln-DT was p-Nitrophenyl-β-D-xylopyranoside
^aThe cell extracts after sonication were heat treated at 75 °C for 30 min, and then cooled in an ice bath, centrifuged at 8000 g for 10 min at 4 °C and the supernatant was kept

ISSN: 1472-6750