Table 1 Summary of microfluidic assembly methods

Kong et al. [22]

Droplet

Polymerase-based (PCA)

500 –1000 bp

1.78 errors/kb

Huang et al. [21]

Microchannel

Polymerase-based (PCA)

760 bp

4.1 errors/kb

Quan et al. [23]

Microarray

Polymerase-based (PCA)

500 – 1000 bp

1.9 errors/kb

Tian et al. [24]

Microarray

Polymerase-based (PAM)

14,500 bp

2.2 errors/kb

Linshiz et al. [48]

Chanel

Exonuclease-based (Gibson) and polymerase-based (IHDC)

754 bp

N/A

Shih et al. [49]

Combined digital and droplet microfluidics

Exonuclease-based assembly of dsDNA (Gibson)

2100 bp

N/A

Tangen et al. [50]

Droplets

Polymerase-based (PCA) and Exonuclease-based (Gibson)

525 bp

N/A

Ben-Yehezkel et al. [18]

Digital microfluidics

Polymerase-based (POP)

800 bp

2.22 errors/kb