Table 1 Primers used in this study
Primers | Sequence(5′-3′) | Description |
|---|---|---|
FAS-RTF | TCCTTTCTATGGTGCTTCCTCA | |
FAS-RTR | ATCTTACCTTGCTGCCGTTCT | FAS RT-qPCR |
Δ5-RTF | TGTCTGGAAGATTCTGGGAGC | |
Δ5-RTR | TTTGGTTGGGCTTGATACGAC | Δ5 RT-qPCR |
Δ6-RTF | TGTTCTGGCAGCAGTGCGGATGG | |
Δ6-RTR | GGCGTGGTGAGTGTTGTGCTTGTCC | Δ6 RT-qPCR |
Δ9-RTF | TCGTTCAGTGGCAGCACAAGA | |
Δ9-RTR | CAAGACGGAGGATAGCAGCATA | Δ9 RT-qPCR |
Δ12-RTF | TGGGTGCTGGCTCACGAGTGT | |
Δ12-RTR | CCAGTGGCCTTGTGGTGCTTC | Δ12 RT-qPCR |
18S-RTF | CGTACTACCGATTGAATGGCTTAG | |
18S-RTR | CCTACGGAAACCTTGTTACGACT | Internal control for RT-qPCR |