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Fig. 2 | BMC Biotechnology

Fig. 2

From: Effective preparation of a monoclonal antibody against human chromogranin A for immunohistochemical diagnosis

Fig. 2

Screening of positive hybridoma clones. a The observation of hybridoma cells cultured in HAT medium. Hybridoma cells were observed after 6 d of fusion (× 100). b The isotype result of positive hybridoma cells. The isotype of monoclonal antibody was determined by isotyping (IgG1, IgG2a, IgG2b, IgG3, IgA, IgM) kit, and the isotype of 4E5 is IgG2b. c Chromosome analysis of 4E5 hybridoma cell (10 × 100). The chromosome number of 4E5 cell line is 102 ± 6

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