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Fig. 1 | BMC Biotechnology

Fig. 1

From: Effective preparation of a monoclonal antibody against human chromogranin A for immunohistochemical diagnosis

Fig. 1

Cloning and Expression of CgA in prokaryotic system. a Identification of the optimized sequence by graphical codon usage analyser (http://gcua.schoedl.de). b Purification and identification of CgA-His. Lane 1: Marker (Blue plus II protein marker), lane 2: the total protein of empty vector pET-28a, lane 3–4: the total protein of pET-28a-CgA in Transetta (DE3), lane 5: the purified CgA-His protein. c Western blot result of CgA-His. Lane 1: Marker (Blue plus II protein marker), lane 2–3: the duplicate for purified CgA-His protein, lane 4–5: the duplicate for western blot result of CgA-His reacted with anti-His6 tag monoclonal antibody

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BMC Biotechnology

ISSN: 1472-6750

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