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Fig. 8 | BMC Biotechnology

Fig. 8

From: UPF1 silenced cellular model systems for screening of read-through agents active on β039 thalassemia point mutation

Fig. 8

Effects of geneticin (G418) on the content of β globin mRNA in β039 and βwt clones. Histograms show the β globin mRNA content in β039 (a) and βwt (b) clones, UPF1 or scramble silenced, and in the original cell lines, β039.m5 and βwt.wt3 respectively, untreated and treated with 400 ng/μl G418. The data were obtained by Real Time qRT-PCR using expression assays for β globin and GAPDH cDNAs, the last of which was used for the normalization. The data represent the averages ± SD of three independent experiments

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BMC Biotechnology

ISSN: 1472-6750

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