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Fig. 7 | BMC Biotechnology

Fig. 7

From: UPF1 silenced cellular model systems for screening of read-through agents active on β039 thalassemia point mutation

Fig. 7

Effects of geneticin (G418) on the β globin production in UPF1- clone 1, scramble clone c and relative original cell line βwt.wt3. After a 3 day incubation with 400 ng/μl G418, cells were labelled with the monoclonal antibody Hemoglobin β-PE and analysed by FACS. Histogram A shows the proportion of β globin producing cells and the fold of that proportion in K562.βwt.wt3, clone 1 and clone c cells, untreated and treated with 400 ng/μl G418. Histogram B shows the fluorescence median of all the samples and its shift after treatment. The data represent the averages ± SD of three independent experiments

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BMC Biotechnology

ISSN: 1472-6750

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