Fig. 6
Effects of geneticin (G418) on the β globin production in UPF1- clone 3, scramble clone a and relative original cell line β039.m5. After a 3 day incubation with 400 ng/μl G418, cells were labeled with the monoclonal antibody Hemoglobin β-PE and analysed by FACS. Histogram a shows the proportion of β globin producing cells and the fold of that proportion in β039.m5, clone 3 and clone a cells, untreated and treated with 400 ng/μl G418. Histogram b shows the fluorescence median of all the samples and its shift after treatment. The data represent the averages ± SD of three independent experiments