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Fig. 3 | BMC Biotechnology

Fig. 3

From: UPF1 silenced cellular model systems for screening of read-through agents active on β039 thalassemia point mutation

Fig. 3

Quantification of the UPF1 mRNA content in UPF1- and scramble clones. Histograms show the UPF1 mRNA content in UPF1- (a) and scramble (b) β039.m5 clones and UPF1- (c) and scramble (d) βwt.wt3 clones and in their original cell lines, β039.m5 and βwt.wt3, used as reference samples. The data were obtained by Real Time qRT-PCR using expression assays for UPF1 and GAPDH cDNAs, the last of which was used for the normalization. The data represent the averages ± SD of three independent determinations

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BMC Biotechnology

ISSN: 1472-6750

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