Fig. 2From: UPF1 silenced cellular model systems for screening of read-through agents active on β039 thalassemia point mutationQuantification of the insertion of pCCL.shRNA-UPF1.WPRE and pCCL.shRNA-scramble.WPRE vectors in the obtained clones. Analysis of the integration of pCCL.shRNA-UPF1.WPRE vector in β039.m5 (a) and βwt.wt3 (c) derived clones and of pCCL.shRNA-scramble.WPRE construct in β039.m5 (b) and βwt.wt3 (d) clones. Histograms show the integration of the lentiviral vectors understood as fold compared to insertions already present in the respective original cells, used as reference samples. The data represent the averages ± SD of three independent determinationsBack to article page