Skip to main content
Fig. 1 | BMC Biotechnology

Fig. 1

From: UPF1 silenced cellular model systems for screening of read-through agents active on β039 thalassemia point mutation

Fig. 1

Cloning strategy for the production of the pCCL.shRNA-UPF1. WPRE lentiviral vector. The pRS-shRNA-UPF1 construct was subjected to enzymatic digestion by the restriction enzymes NaeI and SalI with the purpose to isolate the 410 bp insert containing U6 promoter and UPF1 shRNA.pCCL.PGW vector, as the result of cutting by the SalI and EcoRV endonucleases, was deleted of a region containing the GFP gene and the relative PGK promoter. That region was replaced by the 410 bp insert deriving from the pRS-shRNA-UPF1, obtaining the final vector pCCL.shRNA-UPF1.WPRE

Back to article page