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Fig. 3 | BMC Biotechnology

Fig. 3

From: Development of a baculovirus vector carrying a small hairpin RNA for suppression of sf-caspase-1 expression and improvement of recombinant protein production

Fig. 3

Production of fluorescence proteins using bacmid carrying the Sf-caspase-1 shRNA1 expression cassette. a. Detection of the GFP fluorescence by flow cytometry. Fluorescence histograms (upper panel) and the mean fluorescence intensities (lower panel) are shown. Data were collected from 3 × 104 cells for each sample. b. Determination of the GFP protein levels by Western blot (upper panel). The recombinant GFP was probed by anti-His monoclonal antibody. The loaded total proteins on the PVDF membrane were visualized by coomassie brilliant blue R250 staining, and the GFP band on the stained membrane is indicated by an arrow (lower panel). c. Detection of the DsRed fluorescence by flow cytometry. d. Determination of the DsRed protein levels by Western blot (upper panel). The DsRed band on the stained PVDF membrane is indicated by an arrow (lower panel)

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BMC Biotechnology

ISSN: 1472-6750

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