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Fig. 2 | BMC Biotechnology

Fig. 2

From: Development of a baculovirus vector carrying a small hairpin RNA for suppression of sf-caspase-1 expression and improvement of recombinant protein production

Fig. 2

Suppression of Sf-caspase-1 by baculoviruses expressing shRNAs. a Determination of the transcript levels of Sf-caspase-1 by semi-quantitative RT-PCR. Sf9 cells infected with the baculoviruses expressing the indicated shRNA were analyzed at 2 dpi. The mRNA level of β-actin was detected as the control in each sample. b The relative transcript levels of Sf-caspase-1, which were normalized to the level of actin transcript in each sample. c Analysis of apoptosis by PI staining and flow cytometry. Infected cells were analyzed at 4 dpi. d BV titres of the recombinant baculoviruses

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