Fig. 6

Blood losses or bleeding time in mice subjected to liver laceration or tail transection. Mice were subjected to liver laceration (Panel a) or tail transection protocols (Panels b and c). Blood losses were measured by weighing shed and/or clotted blood (a) or collecting shed blood into water and quantifying hemolysis (b). Continuing bleeding from the transected tail was assessed visually every 30 s for 15 min (c). Mice were injected intravenously with 40 mg/kg body weight of the purified proteins identified on the x axis of each panel. Scatter plots show each data point from a separate mouse in groups of 5 (panel a) or 10 (panels b and c) mice. Horizontal lines depict the mean for each group. Lines above bars show statistical significance between groups as determined by ANOVA with post-tests (p < 0.5, *, p < 0.01, **, p < 0.001, ***)