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Fig. 4 | BMC Biotechnology

Fig. 4

From: A factor XIa-activatable hirudin-albumin fusion protein reduces thrombosis in mice without promoting blood loss

Fig. 4

Time to occlusion of murine ferric chloride-treated carotid arteries. The time to occlusion (TTO) of surgically exposed carotid arteries treated by topical application of 10% (w/vol) ferric chloride, for CD-1 mice receiving saline vehicle (circles) or the two albumin fusion proteins identified on the x axis, in saline (EPR-HV3HSA, squares, and mycHV3HSA, triangles). Mice were injected intravenously with protein doses of 120 mg/kg (Panel a) or 40 mg/kg (Panel b) two minutes prior to application of a ferric chloride-saturated patch (for three minutes). After patch removal blood flow through the artery was monitored for 60 min using ultrasound. Vessels not occluding during the observation period had their TTO recorded as 60 min. Each point in the scatter plots reflects results from a single mouse of a group of 7. Horizontal lines indicate the mean for each group. Lines above groups show statistical significance between groups as determined by ANOVA with post-tests (p < 0.5, *, p < 0.01, **, p < 0.001, ***)

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