Fig. 1
From: Successful production of genome-edited rats by the rGONAD method
Determination of optimal electroporation efficiency for rGONAD. a Scheme of experimental procedures for evaluation of electroporation efficiency using GONAD method. b SZX7 stereomicroscope and Super Electroporator NEPA21. c Tetramethylrhodamine-labelled dextran is instilled into the oviduct lumen using a micropipette inserted through the oviductal wall near the infundibulum. d After the injection, the oviductal regions were covered with a piece of wet paper, and then, electroporation is performed using tweezer-type electrodes. e, f Scheme (e) or diagram (f) of electroporation conditions delivered of by an electroporator. This electroporation parameters were; Poring pulse; 40 V, 5 msec pulse, 50 msec pulse interval, number of pulse 3 times, 10% decay (± pulse orientation) and Transfer pulse; 10 V, 50 msec pulse, 50 msec pulse, number of pulse 6 times, 40% decay (± pulse orientation). g, h Fluorescence analysis of Tetramethylrhodamine-labelled dextran using GONAD method. i-k Graph shows analysis of the percentage of electroporation efficiency in WKY (i), DA (j), and DA x WKY (k). Ova, ovary; Ovi, Oviduct; Ute, uterus. Scale bars: 50 μm (g, h)