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Table 2 E. coli strains and plasmids

From: Soluble expression of an amebic cysteine protease in the cytoplasm of Escherichia coli SHuffle Express cells and purification of active enzyme

Strains or Plasmids

Relevant Genotype or Features

Source

Strains

XL1-Blue MRF′

Δ(mcrA)183 Δ(mcrCB-hsdSMR-mrr)173 endA1 supE44 thi-1 recA1 gyrA96 relA1 lac [F′ proAB lacI q ZΔM15 Tn10 (Tet R )]

Stratagene a

SHuffle Express

fhuA2 [lon] ompT ahpC gal λatt::pNEB3-r1-cDsbC (Spec R , lacI q ) ΔtrxB sulA11 R(mcr-73::miniTn10–Tet S )2 [dcm] R(zgb-210::Tn10 –Tet S ) endA1 Δgor ∆(mcrC-mrr)114::IS10

New England Biolabs

Plasmids

pQE30

Lactose regulation, ColE1 origin, AmpR

Qiagen

pQEhCP1

pQE30-based, recombinant EhCP1

This study

  1. aAgilent Technologies (Santa Clara, CA, USA)