Soluble expression of an amebic cysteine protease in the cytoplasm of Escherichia coli SHuffle Express cells and purification of active enzyme

Table 1 Purification of recombinant EhCP1 (summary)

Purification Step	Total Protein (mg) ^b	Total Activity (units) ^c	Specific Activity (units/mg) ^c	Recovery Yield (%) ^d	Enzyme Purity (%) ^e
Crude lysate ^a	69.75	9918.5	142.2	100.0	2.4
Nickel-affinity	0.81	4731.2	5841.0	47.7	97.0
Gel permeation	0.65	3915.0	6023.0	39.5	100.0

^aObtained from five cell pellets, each from a 200-mL batch (total: 1 L of bacterial culture). ^bProtein concentration determined by Bradford assay. ^cProtease activity measured by the chromogenic assay. ^dRelative amount of total activity at each step as compared to the first. ^eRelative amount of specific activity at each step as compared to the last

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