Fig. 2From: Soluble expression of an amebic cysteine protease in the cytoplasm of Escherichia coli SHuffle Express cells and purification of active enzymeEffect of the substrate concentration on the rate of reaction. a Hydrolysis of Z-Arg-Arg-pNA (0–200 μM) by recombinant EhCP1 (0.05 mg/mL). b Hydrolysis of Z-Arg-Arg-AMC (0–10 μM) by recombinant EhCP1 (0.005 mg/mL). Data represent the mean ± S.E.M. (bars) of 3 independent experimentsBack to article page