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Table 1 Downstream purification process of SacPox and SsoPox 3M

From: High yield production and purification of two recombinant thermostable phosphotriesterase-like lactonases from Sulfolobus acidocaldarius and Sulfolobus solfataricus useful as bioremediation tools and bioscavengers

Purification step

Total protein (mg)

Total Activity (Units)

Specificity (Units/mg)

Enzyme recovery (%)

Purification fold

a

Crude Extract

29150 ± 1457

12748 ± 637

0.44 ± 0.02

n.a

n.a

Thermo-precipitate

8352 ± 250

10793 ± 324

1.29 ± 0.04

84.67 ± 2.54

3.01 ± 0.09

Retentate 100 kDa

4000 ± 80

751 ± 15

0.19 ± 0.004

5.89 ± 0.12

n.a

Permeate 100 kDa

4349 ± 174

10045 ± 402

2.31 ± 0.10

78.80 ± 3.10

5.37 ± 0.20

Retentate 5 kDa

3000 ± 60

9843 ± 196

3.28 ± 0.06

77.21 ± 1.54

7.63 ± 0.15

Permeate 5 kDa

1289 ± 26

201 ± 4

0.15 ± 0.003

1.50 ± 0.03

0.34 ± 0.007

b

Crude Extract

43560 ± 1306

58355 ± 1750

1.34 ± 0.04

n.a

n.a

Thermo-precipitate

12273 ± 613

49421 ± 2471

4.06 ± 0.20

84.69 ± 4.23

3.03 ± 0.15

Retentate 100 kDa

7002 ± 175

3754 ± 94

0.53 ± 0.01

6.43 ± 0.16

0.40 ± 0.10

Permeate 100 kDa

5201 ± 104

45661 ± 913

8.80 ± 0.18

78.25 ± 1.56

6.70 ± 0.13

Retentate 5 kDa

2663 ± 53

45133 ± 902

17.00 ± 0.34

77.34 ± 1.54

13.10 ± 0.26

Permeate 5 kDa

2520 ± 25

480 ± 4.8

0.19 ± 0.002

0.80 ± 0.008

0.15 ± 0.0015

  1. Downstream purification process of SacPox (a) and SsoPox 3 M (b) enzymes from fed-batch fermentation biomasses (600 gcww), induced with 10.0 mM galactose: total protein content, total enzyme activity, specificity, enzyme recovery and purification fold at the different steps