Skip to main content
Fig. 2 | BMC Biotechnology

Fig. 2

From: Phenotype-specific recombinant haptoglobin polymers co-expressed with C1r-like protein as optimized hemoglobin-binding therapeutics

Fig. 2

Haptoglobin and C1r-LP co-expression in FS293F cells. a Coomassie stained reducing SDS-PAGE of rHp1S and rHp2FS produced in transfected FS293F cells. Hp α-chain appears at 12 or 19 kDa and Hp β-chain appears at 47 kDa. Additionally uncleaved proHp1 and proHp2 appear at their expected size of 53 or 57 kDa. b With co-expression of C1r-LP (+) all proHp is efficiently cleaved into its subunits. C1r-LP appears at 68 kDa. The right image shows an anti-8His Western blot, which shows uncleaved proHp in the absence of C1r-LP and smaller Hp β-chain as well as the His-tagged protease in the presence of C1r-LP co-expression. c The Coomassie stained gel of reduced protein samples (left side) illustrates that the cleavage-site mutated (mut) Hp variants remain uncleaved and appear as single proHp bands at their expected size. With non-reduced protein samples polymers of Hp 1–1 and Hp 2–2 can be resolved in wild-type as well as in mutated variants

Back to article page

BMC Biotechnology

ISSN: 1472-6750

Contact us