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Fig. 9 | BMC Biotechnology

Fig. 9

From: Cell-based reference samples designed with specific differences in microRNA biomarkers

Fig. 9

Comparison of average ΔCq (Mix1 – Mix2) across experimental workflows. The y-axis reflects the marginal likelihood (normalized so that the maximum is one). The top row of graphs compares ΔCq results from model 1 across measurement processes (see Fig. 1) using in silico modeled Cq values (see Eq. 1) for each mixture. Black and red lines correspond to samples isolated at BDL and BRL, respectively. Dashed and solid lines correspond to RT-PCR measurements from BDL and BRL, respectively. The bottom row of graphs compares ΔCq results from model 1 between mixture paradigms (see Fig. 2). Red lines correspond to RNA isolated in the BRL then mixed, blue lines correspond to RNA isolated in the BRL after mixing cells. Dashed and solid lines correspond to RT-PCR measurements from BDL and BRL, respectively

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