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Fig. 8 | BMC Biotechnology

Fig. 8

From: Cell-based reference samples designed with specific differences in microRNA biomarkers

Fig. 8

Evaluation of differences in average ΔCq (Mix1–Mix2) between measurement scenarios differing only by measurement or isolation lab. The y-axis reflects the marginal likelihood (normalized so that the maximum is one). Panel a data based on average Cq values inferred for mixtures 1 and 2 derived using Eq. 1 and the average Cq of component cell lines. Isolation lab effect determined by the average difference in ΔCq corresponding to samples isolated at BDL versus those isolated at BRL, for the in silico mixtures. PCR lab effect determined by the average difference in ΔCq corresponding to samples measured at BDL versus those measured at BRL, for the in silico mixtures. Panel b data based on mixtures of RNA isolated and prepared in the BRL. PCR lab effect determined by the average difference in ΔCq corresponding to samples measured at BDL versus those measured at BRL. Black, red, and blue lines correspond to model variations 1, 2, and 3, respectively, as described in Additional file 1

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