Fig. 7
From: Cell-based reference samples designed with specific differences in microRNA biomarkers
Average difference in relative abundance between Mix1 and Mix2 for all measurement scenarios (see Figs. 1 and 2). Panel a is the Bland-Altman plot of relative abundance where ΔCq = Mix1 – Mix2 and Cq values are rescaled relative to the average miR-21 value. Panel c is the Bland-Altman plot of relative abundance of Mix1 and Mix2 compared with ΔΔCq result obtained when using miR-16 as the control [22]. Panels b and d display residuals from panels a and c, respectively (i.e., data for each miRNA are re-centered around corresponding black cross seen in panels a and c, respectively). The black cross denotes center of all values for a particular method of calculation (either ΔCq or ΔΔCq) for each miRNA. Summarized values for each miRNA were compared for each analysis method across the three samples: in silico mixes, RNA mixes, and cell mixes (circles, squares, and triangles, respectively). RNA isolations were performed in either the BDL (black symbols) or BRL (red symbols) and analyzed by RT-PCR in the BDL (open symbols) or BRL (filled symbols). Y-axis is in reverse order to indicate that a negative ΔCq corresponds to more starting material in Mix1 than Mix2