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Fig. 3 | BMC Biotechnology

Fig. 3

From: Novel hemostatic biomolecules based on elastin-like polypeptides and the self-assembling peptide RADA-16

Fig. 3

Expression and solubility analysis of hELPs-RADA-16 expressed in BL21 (DE3) by SDS-PAGE. a Expression of hELPs-RADA-16 expressed in BL21 (DE3). Lane 1: Protein marker; Lane 2: IPTG-induced cells transformed by pET28a (+); Lane3–5: cell lysis of uninduced cells transformed by expression vectors of 36R, 60R and 96R, respectively; Lane 6–8: cell lysis of IPTG-induced cells transformed by expression vectors of 36R, 60R and 96R, respectively. b solubility analysis of hELPs-RADA-16. Lane1: Protein marker; Lane 2–4: supernatant, precipitate and total proteins of ultrasonic cracking cell lysis of BL21 (DE3) transformed by pET28a (+)-hELP36-RADA16; Lane5–7: supernatant, precipitate and total proteins of ultrasonic cracking cell lysis of BL21 (DE3) transformed by pET28a (+)-hELP60-RADA16; Lane8–10: supernatant, precipitate and total proteins of ultrasonic cracking cell lysis of BL21 (DE3) transformed by pET28a (+)-hELP96-RADA16

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BMC Biotechnology

ISSN: 1472-6750

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