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Table 1: Analysis of RNA extracted from mouse tissues

From: Total RNA extraction from tissues for microRNA and target gene expression analysis: not all kits are created equal

Sample Method RNA (ng/μl) 260/280 260/230 RIN miRNA (%)
Brain Isolate II 26.1 (9.5) 2.20 (0.01) 1.28 (0.34) 8.77 (0.21) 5.94 (1.31)
Brain miRVana 211.2 (126.2) 2.02 (0.03) 1.54 (0.61) 8.93 (0.59) 24.35 (3.60)
Brain Norgen total 140.1 (83.7) 2.03 (0.06) 1.86 (0.43) 9.47 (0.38) 17.24 (8.58)
Brain miRNeasy 280 (32.0) 1.98 (0.01) 1.94 (0.12) 9.07 (0.31) 9.45 (1.82)
Brain TRIzol 178.3 (116.4) 1.77 (0.06) 2.40 (0.02) 8.07 (0.51) 9.73 (3.49)
Liver Isolate II 276.7 (167) 2.06 (0.01) 1.47 (0.58) 7.65 (0.49) 12.17 (1.14)
Liver miRVana 720 (244.9) 2.04 (0.03) 1.88 (0.06) 8.03 (0.94) 17.55 (1.22)
Liver Norgen total 904 (306.4) 1.99 (0.03) 1.62 (0.18) 8.10 (2.12) 16.83 (2.22)
Liver miRNeasy 1744 (211.7) 2.01 (0.02) 1.87 (0.25) 7.50 (0.66) 11.06 (3.32)
Liver TRIzol 1824 (483.2) 1.95 (0.01) 2.04 (0.04) 7.23 (0.49) 5.27 (0.47)
Lung Isolate II 132.9 (79.8) 2.06 (0.03) 1.28 (0.39) 7.17 (0.31) 5.39 (1.55)
Lung miRVana 177.3 (22.3) 1.97 (0.02) 1.56 (0.50) 7.95 (1.34) 27.39 (6.16)
Lung Norgen total 128.7 (12.9) 1.96 (0.01) 2.11 (0.09) N.D. 37.48 (2.17)
Lung miRNeasy 262.7 (53.1) 1.97 (0.01) 2.01 (0.01) 6.80 (0.89) 15.42 (0.77)
Lung TRIzol 101.6 (20.4) 1.70 (0.03) 2.49 (0.03) 5.37 (0.67) 14.98 (1.72)
  1. Average measurements from n = 3 mice, with standard deviations in brackets