Table 1: Analysis of RNA extracted from mouse tissues
Sample | Method | RNA (ng/μl) | 260/280 | 260/230 | RIN | miRNA (%) |
|---|---|---|---|---|---|---|
Brain | Isolate II | 26.1 (9.5) | 2.20 (0.01) | 1.28 (0.34) | 8.77 (0.21) | 5.94 (1.31) |
Brain | miRVana | 211.2 (126.2) | 2.02 (0.03) | 1.54 (0.61) | 8.93 (0.59) | 24.35 (3.60) |
Brain | Norgen total | 140.1 (83.7) | 2.03 (0.06) | 1.86 (0.43) | 9.47 (0.38) | 17.24 (8.58) |
Brain | miRNeasy | 280 (32.0) | 1.98 (0.01) | 1.94 (0.12) | 9.07 (0.31) | 9.45 (1.82) |
Brain | TRIzol | 178.3 (116.4) | 1.77 (0.06) | 2.40 (0.02) | 8.07 (0.51) | 9.73 (3.49) |
Liver | Isolate II | 276.7 (167) | 2.06 (0.01) | 1.47 (0.58) | 7.65 (0.49) | 12.17 (1.14) |
Liver | miRVana | 720 (244.9) | 2.04 (0.03) | 1.88 (0.06) | 8.03 (0.94) | 17.55 (1.22) |
Liver | Norgen total | 904 (306.4) | 1.99 (0.03) | 1.62 (0.18) | 8.10 (2.12) | 16.83 (2.22) |
Liver | miRNeasy | 1744 (211.7) | 2.01 (0.02) | 1.87 (0.25) | 7.50 (0.66) | 11.06 (3.32) |
Liver | TRIzol | 1824 (483.2) | 1.95 (0.01) | 2.04 (0.04) | 7.23 (0.49) | 5.27 (0.47) |
Lung | Isolate II | 132.9 (79.8) | 2.06 (0.03) | 1.28 (0.39) | 7.17 (0.31) | 5.39 (1.55) |
Lung | miRVana | 177.3 (22.3) | 1.97 (0.02) | 1.56 (0.50) | 7.95 (1.34) | 27.39 (6.16) |
Lung | Norgen total | 128.7 (12.9) | 1.96 (0.01) | 2.11 (0.09) | N.D. | 37.48 (2.17) |
Lung | miRNeasy | 262.7 (53.1) | 1.97 (0.01) | 2.01 (0.01) | 6.80 (0.89) | 15.42 (0.77) |
Lung | TRIzol | 101.6 (20.4) | 1.70 (0.03) | 2.49 (0.03) | 5.37 (0.67) | 14.98 (1.72) |